Diversity and Distribution of Avifauna in the Northeast of Addis Ababa, Central Ethiopia.

Exploring avian species diversity and distribution patterns is vigorous for conservation efforts in biodiversity-rich countries such as Ethiopia. Compared to other species, birds are relatively well-known and easily observed, making them great markers of productivity or biodiversity. Although bird species are found all across the world, their survival and range have been negatively impacted by habitat loss, fragmentation, and destruction. Thus, the goal of this study is to provide baseline data on avifaunal diversity in the Northeast of Addis Ababa, including species richness, distribution, and relative abundance in various habitats conducted from January 2023 to September 2023 using a stratified sampling design into three habitat types: settlement, farmland, and abattoir. A fixed-width line transect sampling method was used at the farmland and settlement, and a point transect was employed at the abattoir site to collect the bird data. The data were compared using Mann-Whitney and Kruskal-Wallis statistical tests in both seasons and habitat types. A total of 42 bird species belonging to twenty-three families, and nine orders were recorded during the study period. Of these, blue-winged goose and wattled ibis are endemic to Ethiopia. Hooded vultures and White-backed vultures are critically endangered species. The mean abundance of bird species significantly varied in the three habitat types (χ2 = 13.6, df = 2, p=0.001). The abundance of bird species was nonsignificant difference between wet and dry seasons (U = -0.874, p=0.381). The highest diversity (H' = 2.74) was recorded at settlement, and the lowest diversity index (H' = 1.09) was recorded at the abattoir in the dry season. In the wet season, the highest diversity (H' = 2.66) was recorded in the farmland, and the lowest (H' = 1.08) was recorded at the abattoir. The highest evenness (J = 0.94 and J = 0.93) was recorded on the farmland in the wet and dry seasons, respectively. In the study area, urbanization is extremely impacting the environment and altering ecosystem services upon which human civilization depends. Most of the avian species observed in the study area are capable and tolerant of human-induced habitats in the city. Therefore, city planners must consider conserving urban bird species' habitats and feeding sites.

Urinary neopterin reflects immunological variation associated with age, helminth parasitism, and the microbiome in a wild primate.

Neopterin, a product of activated white blood cells, is a marker of nonspecific inflammation that can capture variation in immune investment or disease-related immune activity and can be collected noninvasively in urine. Mounting studies in wildlife point to lifetime patterns in neopterin related to immune development, aging, and certain diseases, but rarely are studies able to assess whether neopterin can capture multiple concurrent dimensions of health and disease in a single system. We assessed the relationship between urinary neopterin stored on filter paper and multiple metrics of health and disease in wild geladas (Theropithecus gelada), primates endemic to the Ethiopian highlands. We tested whether neopterin captures age-related variation in inflammation arising from developing immunity in infancy and chronic inflammation in old age, inflammation related to intramuscular tapeworm infection, helminth-induced anti-inflammatory immunomodulation, and perturbations in the gastrointestinal microbiome. We found that neopterin had a U-shaped relationship with age, no association with larval tapeworm infection, a negative relationship with metrics related to gastrointestinal helminth infection, and a negative relationship with microbial diversity. Together with growing research on neopterin and specific diseases, our results demonstrate that urinary neopterin can be a powerful tool for assessing multiple dimensions of health and disease in wildlife.

A novel statovirus identified in fecal samples from wild geladas in the Ethiopian highlands.

We present a novel statovirus in geladas (Theropithecus gelada), graminivorous primates endemic to the Ethiopian highlands. Using a high-throughput sequencing approach, we identified contiguous sequences in feces from two adult female geladas in the Simien Mountains National Park, Ethiopia, that share similarities to statoviruses. Our phylogenetic analysis of the whole genome, as well as the RNA-dependent RNA polymerase (RdRp) and capsid protein (CP) amino acid sequences, revealed that the gelada statoviruses cluster with those from other primates (laboratory populations of Macaca nemestrina and Macaca mulatta). As the first report of statovirus in wild primates, this finding contributes to our understanding of the phylogenetic and geographic distribution of statoviruses and their hosts.

Genomic signatures of high-altitude adaptation and chromosomal polymorphism in geladas.

Primates have adapted to numerous environments and lifestyles but very few species are native to high elevations. Here we investigated high-altitude adaptations in the gelada (Theropithecus gelada), a monkey endemic to the Ethiopian Plateau. We examined genome-wide variation in conjunction with measurements of haematological and morphological traits. Our new gelada reference genome is highly intact and assembled at chromosome-length levels. Unexpectedly, we identified a chromosomal polymorphism in geladas that could potentially contribute to reproductive barriers between populations. Compared with baboons at low altitude, we found that high-altitude geladas exhibit significantly expanded chest circumferences, potentially allowing for greater lung surface area for increased oxygen diffusion. We identified gelada-specific amino acid substitutions in the alpha-chain subunit of adult haemoglobin but found that gelada haemoglobin does not exhibit markedly altered oxygenation properties compared with lowland primates. We also found that geladas at high altitude do not exhibit elevated blood haemoglobin concentrations, in contrast to the normal acclimatization response to hypoxia in lowland primates. The absence of altitude-related polycythaemia suggests that geladas are able to sustain adequate tissue-oxygen delivery despite environmental hypoxia. Finally, we identified numerous genes and genomic regions exhibiting accelerated rates of evolution, as well as gene families exhibiting expansions in the gelada lineage, potentially reflecting altitude-related selection. Our findings lend insight into putative mechanisms of high-altitude adaptation while suggesting promising avenues for functional hypoxia research.